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mouse mastocytoma cell line p815  (ATCC)


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    ATCC mouse mastocytoma cell line p815
    Mouse Mastocytoma Cell Line P815, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1035 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+mastocytoma+cell+line/P815/pm40961892-57-1-6
    Average 96 stars, based on 1035 article reviews
    mouse mastocytoma cell line p815 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transduction:

    Article Title: PI3Kγ controls leukocyte recruitment, tissue injury, and lethality in a model of graft-versus-host disease in mice.
    Article Snippet: .. P815, a mouse mastocytoma cell line (H-2d, American Type Culture Collection, Manassas, VA, USA), transduced with a lentiviral vector (elongation factor 1 - GFP), was kindly provided by Anna C. Leal and Martin Bonamino (Instituto Nacional de Cancer, Rio de Janeiro, Brazil) and maintained in RPMI/10% FCS at 37°C, and 5% CO2 was used for GVL experiments in vivo. ..

    Article Title: Platelet-activating factor receptor plays a role in the pathogenesis of graft-versus-host disease by regulating leukocyte recruitment, tissue injury, and lethality.
    Article Snippet: PAF is a potent lipid mediator involved in several manifestations of acute inflammation, including leukocyte influx, leukocyte interaction with endothelium, and production of inflammatory cytokines.. The present study evaluated the relevance of PAFR for the pathogenesis of acute GVHD using a model of adoptive transfer of splenocytes from WT or PAFR / C57BL/6J to B6D2F1 mice.. Mice, which received PAFR / splenocytes or treatment with the PAFR antagonist, showed reduced clinical signs of disease and no mortality.

    Article Title: The CCL3/macrophage inflammatory protein-1alpha-binding protein evasin-1 protects from graft-versus-host disease but does not modify graft-versus-leukemia in mice.
    Article Snippet: .. P815, a mouse mastocytoma cell line (H-2d, American Type Culture Collection, Rockville, MD) transduced with a lentiviral vector (EF1aGFP), was kindly provided by A.C. Leal and M. Bonamino from Divisão de Medicina Experimental, Instituto Nacional de Cancer (Rio de Janeiro, Brazil). ..

    Plasmid Preparation:

    Article Title: PI3Kγ controls leukocyte recruitment, tissue injury, and lethality in a model of graft-versus-host disease in mice.
    Article Snippet: .. P815, a mouse mastocytoma cell line (H-2d, American Type Culture Collection, Manassas, VA, USA), transduced with a lentiviral vector (elongation factor 1 - GFP), was kindly provided by Anna C. Leal and Martin Bonamino (Instituto Nacional de Cancer, Rio de Janeiro, Brazil) and maintained in RPMI/10% FCS at 37°C, and 5% CO2 was used for GVL experiments in vivo. ..

    Article Title: Platelet-activating factor receptor plays a role in the pathogenesis of graft-versus-host disease by regulating leukocyte recruitment, tissue injury, and lethality.
    Article Snippet: PAF is a potent lipid mediator involved in several manifestations of acute inflammation, including leukocyte influx, leukocyte interaction with endothelium, and production of inflammatory cytokines.. The present study evaluated the relevance of PAFR for the pathogenesis of acute GVHD using a model of adoptive transfer of splenocytes from WT or PAFR / C57BL/6J to B6D2F1 mice.. Mice, which received PAFR / splenocytes or treatment with the PAFR antagonist, showed reduced clinical signs of disease and no mortality.

    Article Title: The CCL3/macrophage inflammatory protein-1alpha-binding protein evasin-1 protects from graft-versus-host disease but does not modify graft-versus-leukemia in mice.
    Article Snippet: .. P815, a mouse mastocytoma cell line (H-2d, American Type Culture Collection, Rockville, MD) transduced with a lentiviral vector (EF1aGFP), was kindly provided by A.C. Leal and M. Bonamino from Divisão de Medicina Experimental, Instituto Nacional de Cancer (Rio de Janeiro, Brazil). ..

    In Vivo:

    Article Title: PI3Kγ controls leukocyte recruitment, tissue injury, and lethality in a model of graft-versus-host disease in mice.
    Article Snippet: .. P815, a mouse mastocytoma cell line (H-2d, American Type Culture Collection, Manassas, VA, USA), transduced with a lentiviral vector (elongation factor 1 - GFP), was kindly provided by Anna C. Leal and Martin Bonamino (Instituto Nacional de Cancer, Rio de Janeiro, Brazil) and maintained in RPMI/10% FCS at 37°C, and 5% CO2 was used for GVL experiments in vivo. ..

    Article Title: Platelet-activating factor receptor plays a role in the pathogenesis of graft-versus-host disease by regulating leukocyte recruitment, tissue injury, and lethality.
    Article Snippet: PAF is a potent lipid mediator involved in several manifestations of acute inflammation, including leukocyte influx, leukocyte interaction with endothelium, and production of inflammatory cytokines.. The present study evaluated the relevance of PAFR for the pathogenesis of acute GVHD using a model of adoptive transfer of splenocytes from WT or PAFR / C57BL/6J to B6D2F1 mice.. Mice, which received PAFR / splenocytes or treatment with the PAFR antagonist, showed reduced clinical signs of disease and no mortality.

    Incubation:

    Article Title: Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicity
    Article Snippet: .. In the antibody-redirected killing assays, human NKL-FLAG-Tim-3 cells, fresh PBMCs, or overnight IL-2 (200 U/mL)–activated PBMCs were used as effectors and were incubated for 4 hours with the 51 Cr-labeled mouse FcR + P815 cells, a mouse mastocytoma cell line (ATCC). .. P815 cells were coated with 10 μg/mL antibodies against different receptors: anti-CD16 (clone Leu11a; BD Biosciences), anti-2B4 (clone C1.7; a gift from Dr G. Trinchieri, National Cancer Institute), anti-NKG2D (clone 149810; R&D Systems), anti–Tim-3 (clone 344836; R&D Systems), anti–Tim-3 (clone 344801; a generous gift from Dr J. P. Houchins, R&D Systems), anti-CD94 (clone DX22), anti-CD56 (clone My31.13), or against the FLAG epitope tag (clone M2; Sigma-Aldrich).

    Cell Culture:

    Article Title: Lenalidomide down regulates the production of interferon-gamma and the expression of inhibitory cytotoxic receptors of human Natural Killer cells.
    Article Snippet: Contents lists available at ScienceDirect Cellular Immunology journal homepage: www.elsevier .com/locate /yc imm Lenalidomide down regulates the production of interferon-c and the expression of inhibitory cytotoxic receptors of human Natural Killer cells Nicolas Dauguet, Jean-Jacques Fournié, Rémy Poupot, Mary Poupot * INSERM, U.563, Centre de Physiopathologie de Toulouse-Purpan, Toulouse, F-31300, France Université Paul-Sabatier, Toulouse, F-31400, France a r t i c l e i n f o Article history: Received 21 April 2010 Accepted 14 June 2010 Available online 17 June 2010 Keywords: Cell surface molecules Cytotoxicity Immunomodulation Natural Killer cells Lenalidomide 0008-8749/$ - see front matter 2010 Elsevier Inc. A doi:10.1016/j.cellimm.2010.06.003 * Corresponding author.. Address: INSERM 563 – C Toulouse-Purpan, CHU Purpan, BP 3028, 31024 Toulo 562 744 558.. E-mail address: mary.poupot@inserm.fr (M. Poupo 1 Abbreviations used: CLL, Chronic Lymphocytic Leuk IFN-c, interferon-c; IL-2, interleukin-2; KIR(s), Kill Receptor(s); LPS, LipoPolySaccharide; MFI, Mean Fl Metastatic Renal Cell Cancer; MESF, Molecules of Equi mAb, monoclonal antibody; MM, Multiple Myeloma dromes; NCR, Natural Cytotoxicity Receptors; NK, Na Blood Mononuclear Cells; TNF-a, Tumor Necrosis Facto a b s t r a c t Lenalidomide, a daughter molecule of Thalidomide, and IMIDs are immunomodulatory drugs that have been described as having immunomodulatory properties and anti-tumor activity.

    other:

    Article Title: Stobadine inhibits doxorubicin-induced apoptosis through a caspase-9 dependent pathway in P815 mastocytoma cells.
    Article Snippet: Doxorubicin (DOXO), a widely used chemotherapeutic agent, induces apoptosis in transformed and non-transformed cells.. The apoptotic effect of DOXO has been linked to the generation of reactive oxygen species (ROS).. Antioxidants may be effective in the prevention of DOX-induced apoptosis.



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    p815 mouse mastocytoma cell line  (DSMZ)
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    DSMZ p815 mouse mastocytoma cell line
    a Heatmaps of normalized and scaled genes distinguishing cytotoxic (Tc1 and Tc17 + 1) and noncytotoxic CD8 + and CD4 + T-cell subsets (Tc2, Tc17, Tc22, Th1, Th2, Th17, Th17 + 1, and Th22). b Representative dot plot of IL-6R and SLAMF7 expression of human lymphocytes. c , d Frequency of IL-6R and SLAMF7 among human naive CD45RA + CCR7 + , EMRA CD45RA + CCR7 − , and memory CD45RA − CD8 + (black circles) and CD4 + (gray circles) T-cell subsets. Mean ± SEM. e Co-expression of SLAMF7 with granzyme B, perforin, and IFN-γ in polyclonally activated human CD8 + and CD4 + T cells. f Redirected killing of α-CD3-coated <t>p815</t> mouse <t>mastocytoma</t> cell line after 6 h cocultivation with sorted CD45RA − CD4 + or CD8 + T cells expressing either SLAMF7 or IL-6R. Lysis is calculated by the reduction in the % of viable p815 + cells in the presence of α-CD3 compared to uncoated controls ( n = 3). Mean ± SEM. Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001. g Abundance of different memory CD8 + and CD4 + T-cell subsets in human tonsils, lung, and bone marrow. Mean ± SEM. h Human EMRA CD8 + (CD45RA + CCR7 − ), helper CD8 + memory (CD45RA − CXCR3 − CCR4 + ), cytotoxic CD8 + memory (CD45RA − CXCR3 + CCR4 − ), helper CD4 + memory (CD45RA − CD28 + ), and cytotoxic CD4 + memory (CD45RA − CD28 − CD57 + ) T cells were sorted and RUNX3 expression assessed. Numbers in plot indicate the MFI. i Granzyme B and CD40L co-expression of sorted and polyclonally activated human helper CD4 + memory (CD45RA − CD28 + ) and cytotoxic CD4 + (CD45RA − CD28 − CD57 + ) T cells compared to unstimulated control.
    P815 Mouse Mastocytoma Cell Line, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a Heatmaps of normalized and scaled genes distinguishing cytotoxic (Tc1 and Tc17 + 1) and noncytotoxic CD8 + and CD4 + T-cell subsets (Tc2, Tc17, Tc22, Th1, Th2, Th17, Th17 + 1, and Th22). b Representative dot plot of IL-6R and SLAMF7 expression of human lymphocytes. c , d Frequency of IL-6R and SLAMF7 among human naive CD45RA + CCR7 + , EMRA CD45RA + CCR7 − , and memory CD45RA − CD8 + (black circles) and CD4 + (gray circles) T-cell subsets. Mean ± SEM. e Co-expression of SLAMF7 with granzyme B, perforin, and IFN-γ in polyclonally activated human CD8 + and CD4 + T cells. f Redirected killing of α-CD3-coated p815 mouse mastocytoma cell line after 6 h cocultivation with sorted CD45RA − CD4 + or CD8 + T cells expressing either SLAMF7 or IL-6R. Lysis is calculated by the reduction in the % of viable p815 + cells in the presence of α-CD3 compared to uncoated controls ( n = 3). Mean ± SEM. Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001. g Abundance of different memory CD8 + and CD4 + T-cell subsets in human tonsils, lung, and bone marrow. Mean ± SEM. h Human EMRA CD8 + (CD45RA + CCR7 − ), helper CD8 + memory (CD45RA − CXCR3 − CCR4 + ), cytotoxic CD8 + memory (CD45RA − CXCR3 + CCR4 − ), helper CD4 + memory (CD45RA − CD28 + ), and cytotoxic CD4 + memory (CD45RA − CD28 − CD57 + ) T cells were sorted and RUNX3 expression assessed. Numbers in plot indicate the MFI. i Granzyme B and CD40L co-expression of sorted and polyclonally activated human helper CD4 + memory (CD45RA − CD28 + ) and cytotoxic CD4 + (CD45RA − CD28 − CD57 + ) T cells compared to unstimulated control.

    Journal: Nature Communications

    Article Title: SLAMF7 and IL-6R define distinct cytotoxic versus helper memory CD8 + T cells

    doi: 10.1038/s41467-020-19002-6

    Figure Lengend Snippet: a Heatmaps of normalized and scaled genes distinguishing cytotoxic (Tc1 and Tc17 + 1) and noncytotoxic CD8 + and CD4 + T-cell subsets (Tc2, Tc17, Tc22, Th1, Th2, Th17, Th17 + 1, and Th22). b Representative dot plot of IL-6R and SLAMF7 expression of human lymphocytes. c , d Frequency of IL-6R and SLAMF7 among human naive CD45RA + CCR7 + , EMRA CD45RA + CCR7 − , and memory CD45RA − CD8 + (black circles) and CD4 + (gray circles) T-cell subsets. Mean ± SEM. e Co-expression of SLAMF7 with granzyme B, perforin, and IFN-γ in polyclonally activated human CD8 + and CD4 + T cells. f Redirected killing of α-CD3-coated p815 mouse mastocytoma cell line after 6 h cocultivation with sorted CD45RA − CD4 + or CD8 + T cells expressing either SLAMF7 or IL-6R. Lysis is calculated by the reduction in the % of viable p815 + cells in the presence of α-CD3 compared to uncoated controls ( n = 3). Mean ± SEM. Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001. g Abundance of different memory CD8 + and CD4 + T-cell subsets in human tonsils, lung, and bone marrow. Mean ± SEM. h Human EMRA CD8 + (CD45RA + CCR7 − ), helper CD8 + memory (CD45RA − CXCR3 − CCR4 + ), cytotoxic CD8 + memory (CD45RA − CXCR3 + CCR4 − ), helper CD4 + memory (CD45RA − CD28 + ), and cytotoxic CD4 + memory (CD45RA − CD28 − CD57 + ) T cells were sorted and RUNX3 expression assessed. Numbers in plot indicate the MFI. i Granzyme B and CD40L co-expression of sorted and polyclonally activated human helper CD4 + memory (CD45RA − CD28 + ) and cytotoxic CD4 + (CD45RA − CD28 − CD57 + ) T cells compared to unstimulated control.

    Article Snippet: The p815 mouse mastocytoma cell line was purchased from DSMZ and cultivated in RPMI 1640 medium supplemented with 100 U/mL penicillin, 0.1 mg/mL streptomycin, and 10% inactivated FCS (PAA) at 0.5–5 × 105 cells/mL.

    Techniques: Expressing, Lysis, Control